four-parameter standard curve Search Results


four-parameter standard curve  (GraphPad Software Inc)
90
GraphPad Software Inc four-parameter standard curve
Four Parameter Standard Curve, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/four-parameter standard curve/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
four-parameter standard curve - by Bioz Stars, 2026-03
90/100 stars
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prism 9.5.1 standard curve interpolation (four parameter) tool  (GraphPad Software Inc)
90
GraphPad Software Inc prism 9.5.1 standard curve interpolation (four parameter) tool
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Prism 9.5.1 Standard Curve Interpolation (Four Parameter) Tool, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prism 9.5.1 standard curve interpolation (four parameter) tool/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prism 9.5.1 standard curve interpolation (four parameter) tool - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
standard curve generated by four parameter logistic curve fit using sigmastat 3.5  (SYSTAT)
90
SYSTAT standard curve generated by four parameter logistic curve fit using sigmastat 3.5
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Standard Curve Generated By Four Parameter Logistic Curve Fit Using Sigmastat 3.5, supplied by SYSTAT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard curve generated by four parameter logistic curve fit using sigmastat 3.5/product/SYSTAT
Average 90 stars, based on 1 article reviews
standard curve generated by four parameter logistic curve fit using sigmastat 3.5 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
standard curve analysis (four parameter logistic curve) feature of sigmaplot  (SYSTAT)
90
SYSTAT standard curve analysis (four parameter logistic curve) feature of sigmaplot
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Standard Curve Analysis (Four Parameter Logistic Curve) Feature Of Sigmaplot, supplied by SYSTAT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard curve analysis (four parameter logistic curve) feature of sigmaplot/product/SYSTAT
Average 90 stars, based on 1 article reviews
standard curve analysis (four parameter logistic curve) feature of sigmaplot - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
fourparameter logistic standard curve  (GraphPad Software Inc)
90
GraphPad Software Inc fourparameter logistic standard curve
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Fourparameter Logistic Standard Curve, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fourparameter logistic standard curve/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
fourparameter logistic standard curve - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.

Journal: Scientific Reports

Article Title: Engineering a GPCR-based yeast biosensor for a highly sensitive melatonin detection from fermented beverages

doi: 10.1038/s41598-024-68633-y

Figure Lengend Snippet: Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.

Article Snippet: Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool.

Techniques: Expressing, Over Expression, Derivative Assay, Fluorescence

Modifications on original strain affecting melatonin sensing properties. A comparison of the dose–response between original biosensor strain and after increasing MTNR1A receptor number (yRB1002) ( A ), after increasing MTNR1A and adding one extra copy of the reporter cassette (yRB1012) ( B ) and after increasing MTNR1A and integrating reporter cassette multiple times (yRB1022) ( C ). Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.

Journal: Scientific Reports

Article Title: Engineering a GPCR-based yeast biosensor for a highly sensitive melatonin detection from fermented beverages

doi: 10.1038/s41598-024-68633-y

Figure Lengend Snippet: Modifications on original strain affecting melatonin sensing properties. A comparison of the dose–response between original biosensor strain and after increasing MTNR1A receptor number (yRB1002) ( A ), after increasing MTNR1A and adding one extra copy of the reporter cassette (yRB1012) ( B ) and after increasing MTNR1A and integrating reporter cassette multiple times (yRB1022) ( C ). Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.

Article Snippet: Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool.

Techniques: Comparison, Fluorescence

Screening of 101 yeast strains. Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool. Melatonin concentration values are shown as mean ± SD of triplicates. 17 of the 101 strains assayed were excluded from interpolation due to lack of enough signal.

Journal: Scientific Reports

Article Title: Engineering a GPCR-based yeast biosensor for a highly sensitive melatonin detection from fermented beverages

doi: 10.1038/s41598-024-68633-y

Figure Lengend Snippet: Screening of 101 yeast strains. Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool. Melatonin concentration values are shown as mean ± SD of triplicates. 17 of the 101 strains assayed were excluded from interpolation due to lack of enough signal.

Article Snippet: Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool.

Techniques: Fluorescence, Concentration Assay